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solid phase sandwich enzyme linked immunosorbent assay kit  (R&D Systems)


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    R&D Systems solid phase sandwich enzyme linked immunosorbent assay kit
    Solid Phase Sandwich Enzyme Linked Immunosorbent Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solid+phase+sandwich+elisa+kits/Human+Proprotein+Convertase+9%2FPCSK9+DuoSet+ELISA/pm41469658-61-7-20
    Average 94 stars, based on 36 article reviews
    solid phase sandwich enzyme linked immunosorbent assay kit - by Bioz Stars, 2026-09
    94/100 stars

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    Sandwich ELISA:

    Article Title: A physiologically relevant culture platform for long-term studies of in vitro gingival tissue.
    Article Snippet: There is a clinical need to understand the etiologies of periodontitis, considering the growing socioeconomic impact of the disease.. Despite recent advances in oral tissue engineering, experimental approaches have failed to develop a physiologically relevant gingival model that combines tissue organization with salivary flow dynamics and stimulation of the shedding and non-shedding oral surfaces.. Herein, we develop a dynamic gingival tissue model composed of a silk scaffold, replicating the cyto-architecture and oxygen profile of the human gingiva, along with a saliva-mimicking medium that reflected the ionic composition, viscosity, and non-Newtonian behavior of human saliva.

    Article Title: Bison Meat has a Lower Atherogenic Risk than Beef in Healthy Men
    Article Snippet: Oxidized LDL concentrations were measured using an enzyme immunoassay (Mercodia, kit 10-1143-01, Uppsala, Sweden). .. High sensitivity IL-6, IL-10 and TNF-alpha as well as PAI-1 concentrations were measured using a solid phase sandwich ELISA kits (kits HS600B, D100B, DTA00C, DSE100 respectively, R&D Systems: Minneapolis, MN). .. Protein carbonyl were measured by a protein Carbonyl ELISA (kit NWK-PCK01, Northwest Life Science Specialities, LLC., Vancouver, Washington).

    Article Title: Loss of lung mesenchymal identities in culture and a screening approach to identify restorative cues
    Article Snippet: .. Levels of secreted ANGPT1 and IGFBP-4 in conditioned media were determined using colorimetric, Solid Phase Sandwich ELISA kits (R&D Systems; ANGPT1: DANG10 (RRID: AB_2893027), IGFBP4: DY804) according to manufacturer’s protocols. ..

    Article Title: The CCL28 levels are elevated in the serum of patients with irritable bowel syndrome and associated with the clinical symptoms
    Article Snippet: .. The concentration of CCL28 levels were examined using solid-phase sandwich ELISA kits according to the manufacturer’s instructions (Cat# DY717, R & D Systems, San Diego, CA). ..

    Concentration Assay:

    Article Title: The CCL28 levels are elevated in the serum of patients with irritable bowel syndrome and associated with the clinical symptoms
    Article Snippet: .. The concentration of CCL28 levels were examined using solid-phase sandwich ELISA kits according to the manufacturer’s instructions (Cat# DY717, R & D Systems, San Diego, CA). ..



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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic <t>factor</t> <t>VEGF</t> by microtissues was quantified in cell culture supernatants using <t>ELISA</t> over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.
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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic <t>factor</t> <t>VEGF</t> by microtissues was quantified in cell culture supernatants using <t>ELISA</t> over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.
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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic factor VEGF by microtissues was quantified in cell culture supernatants using ELISA over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.

    Journal: Advanced Healthcare Materials

    Article Title: siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA

    doi: 10.1002/adhm.202504773

    Figure Lengend Snippet: Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic factor VEGF by microtissues was quantified in cell culture supernatants using ELISA over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.

    Article Snippet: The amount of VEGF secreted was quantified by using a solid phase sandwich ELISA Kit (Human VEGF DuoSet ELISA, R&D Systems, Wiesbaden, Germany) according to the manufacturer's protocol.

    Techniques: Gene Expression, Activity Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Small Interfering RNA